世界*实验材料供应商 Expedeon 上海金畔生物为其中国代理, Expedeon 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Expedeon 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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expedeon一直为科学界提供高价值、低成本的蛋白研究产品自1999。我们全系列的预制聚丙烯酰胺凝胶,蛋白质污渍,亲和树脂,和蛋白质quantitiation检测,将提高你的蛋白质的鉴定与分析。我们的蛋白质发现线样品制备和质谱蛋白质分离产品是蛋白质组分析的前沿。
SYGNIS和Expedeon通过为科学界提供的,的基因组学和蛋白质组学工具,共享创新和服务的愿景。通过加入SYGNIS的基因组研发专长与Expedeon的蛋白质组学工具和消耗品,Expedeon将能够在涉及单细胞基因组学,大规模蛋白质组学和两者之间的工作流程中提供各种创新产品。
我们在英国,美国,西班牙,德国和新加坡设有办事处,实验室和制造工厂。我们生产和销售预制丙烯酰胺凝胶,蛋白质污渍,定量试剂,纯化树脂,蛋白质溶解试剂和核酸扩增和操纵产品。Expedeon致力于为基因组研究(特别是单细胞应用)和蛋白质组学研究提供高质量,有效和快速的解决方案。
试剂名称: | InstantBlue蛋白电泳染色剂 |
英文名称: | InstantBlue |
国产/进口: | 进口 |
产地/品牌: | Expedeon |
规格: | 1L |
货号: | ILSB1L-1L |
InstantBlue 是已配制好可直接使用的蛋白凝胶电泳染色剂,速度快,灵敏度高, 无毒,效果好。
Expedeon 的 InstantBlue 是*真正的实现一步考马斯凝胶染色。只需简单的从电泳槽中取出凝胶、放入染色剂中,就可离开了。无需洗涤、微波加热或脱色。所有条带将在 15 分钟内可见。凝胶可以无限期留在 InstantBlue试剂内, 只有蛋白质被染色,不会增加背景染色。InstantBlue 是无毒的并且与质谱分析兼容。
超快
15分钟内即可看到结果
简单
一步即可
l 染色前不需要清洗
l 染色前不需要固定
l 不需要加热/微波
l 不需要脱色
灵敏
≥5ng 蛋白/带
如果放置时间太长,没有过染的风险
使能够
可用于质谱
Edman Sequencing
安全
无毒
无甲醇
废液可直接排放
与其它染色剂相比,*特色:
InstantBlue Features & Benefits
• Ultra-fast staining – Results in 15 minutes or less
• Single step procedure – No washing, fixing, microwaving or destaining
• High sensitivity – 5ng bands detectable*
• Clear background – High signal/noise ratio
• Flexible – No over staining
• Quantitative – Batch to batch consistency
• Efficient – Only 25ml per gel
• Safe composition – Non-toxic. No fume hood or solvent disposal required
• Methanol free – No gel shrinkage or protein methylation
• Acetic acid free – No protein aceylation
• MS Compatible 1 – Destainable. No residual methylation or acetylation
Additional Information
Storage | +4C |
---|---|
Shelf Life | 1 Year from receipt |
InstantBlue is ready for use straight out the bottle and comes in convenient pre-mixed 1 litre volumes. Its unique patented mechanism begins to stain in moments and results are achieved within 15 minutes. Some premade and traditional home-made Coomassie R-250 stains can take 3 hours or more to fully stain gels.
There’s no need to wash, fix, microwave or destain gels when using InstantBlue. Homemade stains often require all these steps and most premade solutions require at least one. InstantBlue uses a one-step protocol and can be fully destained using standard procedures. InstantBlue’s acetic acid free formulation doesn’t methylate or acetylate protein during staining making it fully compatible with mass spectrometry.
Unparalleled sensitivity allows protein levels as low as 5 ng per band to be detected (BSA) when staining overnight. This is thanks to InstantBlue’s protein specific staining which gives a clear background, improving the signal/noise ratio of the stain. Gels can be left in InstantBlue for weeks and still come out with a crystal clear background, all without the need for destaining.
InstantBlue is non-toxic and sink disposable due to its methanol free composition. Other stains require solvent disposal procedures and can require microwaving which produces dangerous fumes. InstantBlue’s effective staining and safe composition means there’s no need for microwaving, solvent disposal, or fume hoods. The lack of methanol also means no gel shrinkage.
Economical staining with only 25ml per mini-gel allows you to use less InstantBlue. Homemade solutions and some pre-mixed solutions require about 50-100ml of stain per gel. InstantBlue will stain at least 40 mini-gels per bottle.