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Carboxyl Microspheres

Our high degree of specialization and expertise in microparticle technology enabled us to concentrate on all aspects of carboxyl microspheres. An enormous amount of research work has been focused on the development of every possible carboxyl microsphere for diagnostic assays. Within each size, we offer different levels of functionality to provide a range of selectivity, sensitivity, and specificity as desired for each application.

  • More than 100 products – various sizes and surface charge densities
  • Optimized levels of carboxyl and sulfate groups for maximum coupling efficiency
  • Very uniform surface morphology and even distribution of functional groups
  • Can be used without precleaning for most applications
  • Highly uniform, stable, and reproducible
  • Fluorescent and Dyed microspheres: Available in selected sizes

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
20 CB0020I 250 173.0
25 CB0025G 230 165.0
30 CB0030F 190 166.4
35 CB0035G 230 117.8
42 CB0042G 200 112.9
46 CB0046I 260 79.3
50 CB0050I 260 73.0
52 CB0052G 230 79.3
62 CB0062F 200 76.5
71 CB0071D 120 111.3
72 CB0072F 180 73.2
81 CB0081D 140 83.6
94 CB0094D 130 77.6
97 CB0097C 100 97.8
98 CB0098D 115 84.2
100 CB0100E 170 55.8
104 CB0104D 110 82.9
105 CB0105D 110 82.1
115 CB0115D 110 75.0
120 CB0120F 190 41.6
122 CB0122C 80 97.2
127 CB0127D 120 62.2
130 CB0130E 170 42.9
135 CB0135F 200 35.1
136 CB0136C 90 77.5
136 CB0136E 170 41.0
140 CB0140E 170 39.9
146 CB0146D 105 61.9
160 CB0160B 65 91.2
172 CB0172E 160 34.5
193 CB0193F 190 25.9
195 CB0195B 65 74.8
201 CB0201E 165 28.6
206 CB0206D 120 38.4
207 CB0207E 170 27.0
210 CB0210C 85 53.1
213 CB0213E 116 27.8
226 CB0226C 85 49.4
230 CB0230D 125 33.0
258 CB0258D 110 33.4
267 CB0267D 105 33.8
286 CB0286C 90 36.9
297 CB0297C 100 31.9
300 CB0300B 70 45.2
309 CB0309B 70 43.9
313 CB0313C 100 30.3
350 CB0350B 70 38.7
358 CB0358B 60 44.2
376 CB0376C 80 31.5
385 CB0385B 65 37.9
394 CB0394B 70 34.4
408 CB0408B 60 38.7
419 CB0419D 110 20.6
420 CB0420C 80 28.2
600 CB0600D 105 15.1
614 CB0614B 70 22.1
840 CB0840B 70 16.1
930 CB0930D 100 10.2
1020 CB1020B 70 13.3
1120 CB1120C4 60 14.1
1730 CB1730C4 25 21.9
We also make it to your exact specs.

Near-Soap-Free? Carboxyl Microspheres

PolyMicrospheres innovative process technology has led to the development of a novel product line, “Near-Soap-Free” carboxyl microspheres with high MW. The surface chemistry is similar to conventional microspheres, very uniform and stable, but with very little surfactants.

  • Better than soap-free microparticles with no residual monomer and with optimum amount of sulfate groups
  • The very little surfactant level with a novel process technology provides stability and uniformity throughout the polymerization
  • Surfactant levels of the products are very low (from 0.01% to 0.05%) and do not require clean up
  • Optimized levels of carboxyl groups and uniform surface morphology for maximum coupling efficiency
  • Highly uniform, stable, and reproducible
  • Fluorescent and Dyed microspheres: Available in selected sizes
  • We also make it to your exact specs

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
120 NS0120F 200 39.5
135 NS0135G 220 31.9
195 NS0195F 200 24.3
207 NS0207F 185 24.8
300 NS0300D 110 28.7
315 NS0315C 100 30.1
380 NS0380C 80 31.2
420 NS0420C 80 28.2
600 NS0600D 110 14.4

SpaceLink? Carboxyl Microspheres

SpaceLink? Carboxyl Microspheres are perfect immunodiagnostic reagent particles with a spacer-linker already attached to the microspheres. The length of the flexible spacer arm is ~10A°. The end of the spacer arm has a carboxyl group for covalent attachment of ligands. The second generation of SpaceLink microspheres have been developed and tested at ?-sites for best coupling efficiency.

The benefits of SpaceLink? Carboxyl microspheres include:

  • Almost all of the ligands coupled to the microspheres will be in an active form
  • Conformational changes or orientation problems of the ligands are minimized when the ligands are located away from the surface of the particles
  • Higher level of sensitivity compared to direct surface coupling; Lesser amount of ligand needed to get the required sensitivity
  • The flexible spacer arms improve the kinetics of agglutination reactions and reactivity of each coupled ligand by keeping the ligands away from the surface of the particles
  • Antigen/antibody recognition sites line up much more easily
  • The flexible spacer arms greatly reduce steric hindrance problems
  • We also make it to your exact specs
  • Also available: SpaceLink Hydroxyl microspheres

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
95 SL0095C 60 166.4
107 SL0107C 90 98.5
175 SL0175C 60 90.3
200 SL0200C 55 86.2
240 SL0240C 55 71.9
280 SL0280C 45 75.3
300 SL0300C 45 70.3
382 SL0382C 40 62.1
591 SL0591C 40 40.1
1010 SL1010C 35 26.8

Specialty Carboxyl with steric effects

These microspheres are designed with bulky groups to minimize passive adsorption and reduce non-specific binding.

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
165 SE0165E3X 160 35.9
296 SE0296C2X 90 35.6
310 SE0310C6X 82 37.3

Hydrophilic Carboxyl Microspheres

These microspheres are designed to enhance stability and reactivity.

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
100 HC0100G 230 41.2
182 HC0182E 145 35.9
200 HC0200E 170 27.9
300 HC0300C 90 35.1

Omni-Bind? Lipophilic Carboxyl Microspheres

Our innovative process technology and formulations have led to the development of novel lipophilic (lipid incorporated/coated) carboxyl microspheres. These microspheres have been developed with optimized levels of different lipids to increase the coupling efficiency of various proteins.

  • The lipophilic microspheres contour to the conformation of the proteins for better coupling efficiency and improved visibility of the agglutinates
  • We also make it to your exact specs

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
213 LP0213CA 90 49.5
242 LP0242CA 90 43.6
308 LP0308CA 115 26.8

Low-Density Carboxyl Microspheres

Our innovative process technology and formulations have led to the development of novel low-density carboxyl microspheres.

  • Optimized levels of carboxyl and sulfate groups for maximum coupling efficiency
  • Highly uniform, stable, and reproducible
  • Designed to reduce settling
Diameter, nm Catalog Number Density Carboxyl μEquiv/gram Parking Area
106 LD0106C3 1.03 170 52.6
129 LD0129C2 1.02 202 36.4
153 LD0153C2 1.02 150 41.3
298 LD0298C3 1.03 80 39.8
302 LD0302C2 1.02 80 39.3
306 LD0306C2 1.02 90 34.4
310 LD0310C1 1.01 82 37.3
388 LD0388C2 1.02 120 20.4
407 LD0407C2 1.02 110 21.2
421 LD0421C2 1.02 75 30.0
489 LD0489C2 1.02 124 15.6
1117 LD1117C4 1.035 59 14.4
1730 LD1730C4 1.035 26 21.1

Non-polystyrene Carboxyl microspheres

These microspheres are designed to reduce non-specific binding and are easy to centrifuge.

Diameter, nm Catalog Number Carboxyl μEquiv/gram Parking Area
98 NP0098D 105 92.2
168 NP0168C 164 34.4
194 NP0194C 148 33.0
298 NP0298C 72 44.2
402 NP0402B 62 38.1

High-Density Carboxyl Microspheres

Diameter, nm Catalog Number Density Carboxyl μEquiv/gram Parking Area
100 HD0100C2 1.19 102 93.0
170 HD0170C2 1.19 160 34.9
200 HD0200C2 1.19 150 31.6
300 HD0300C2 1.19 70 45.2
400 HD0400C3 1.19 60 39.5

Soap-Free Carboxyl Microspheres

Our distinctive soap-free carboxyl microspheres have the following unique features:

  • Optimum sulfate and carboxyl groups to stabilize the microparticles
  • Innovative process technology and formulation provide stability and uniformity throughout the polymerization
  • Optimized levels of carboxyl groups and uniform surface morphology for maximum coupling efficiency. No excess sulfate groups to interfere with coupling chemistry.
  • No residual monomer
  • Very uniform, stable, and reproducible
  • Fluorescent and Dyed microspheres: Available in selected sizes
  • We also make it to your exact specs

Diameter, nm Catolog Number Carboxyl μEquiv/gram Parking Area

100 SF0100C 170 55.8
120 SF0120C 190 41.6
135 SF0135C 200 35.1
146 SF0146C 105 61.9
172 SF0172C 160 34.5
195 SF0195C 200 24.3
202 SF0202C 165 28.5
300 SF0300C 110 28.7
380 SF0380C 80 31.2
600 SF0600C 110 14.4

Fluorescent / Dyed Carboxyl Microspheres

Standard Colors: Fluorescent Yellow, Blue, Red, Hot Pink, Pink, Violet, Coral, Lavender, and other colors

We can dye any product in this catalog to the color of your choice.

Ready-to-Couple Products

Ready-to-Couple? Chloromethyl Microspheres

PolyMicrospheres proprietary formulations and innovative process technology have led to the development of highly stable and very uniform chloromethyl microspheres. These ready-to-couple microspheres are perfect diagnostic particles with activated groups already attached to the microspheres.

  • Preactivated and ready for covalent coupling
  • Just adjust the pH and stir at room temp
  • High stability and long shelf life
  • Simple one-step coupling chemistry
  • Optimized levels of chloromethyl groups and uniform surface morphology for maximum coupling efficiency
  • Highly uniform, stable, and reproducible
  • We also make it to your exact specs
  • Refer to the coupling procedure

Note: Chloromethyl is the best choice for simple one-step covalent coupling to microspheres

Diameter, nm Catalog Number
95 CL009
195 CL0195B
246 CL0246B
280 CL0280B
304 CL0304B
360 CL0360B
405 CL0405B

Ready-to-Couple? Epoxy Microspheres

PolyMicrospheres proprietary formulations and innovative process technology have led to the development of preactivated and very stable epoxy microspheres for easy covalent coupling. The epoxy microspheres are ready-to-couple diagnostic particles with activated groups already attached to the microspheres.

  • Preactivated and ready for covalent coupling
  • Fast and easy one-step coupling
  • Optimized levels of epoxy groups and uniform surface morphology for maximum coupling efficiency
  • Highly uniform, stable, and reproducible
  • We also make it to your exact specs
  • Refer to the coupling procedure

Diameter, nm Catalog Number Type
105 EP0105B Epoxy-II
228 EP0228B Epoxy-II
314 EP0314B Epoxy-II
383 EP0383B Epoxy-II
585 EP0585B Epoxy-II

Plain-surface Products

Polystyrene Microspheres

The polystyrene microspheres are specifically designed for diagnostic assays and optimized for superior performance. Our proprietary formulations and process techniques have resulted in uniform, stable, and reproducible microspheres.

  • Optimized levels of sulfate groups to stabilize the microparticles
  • Very uniform surface morphology for best passive adsorption
  • Can be used without precleaning for most applications
  • Fluorescent and Dyed microspheres: Available in selected sizes

Diameter, nm Catalog Number
45 PS0045P
50 PS0050P
65 PS0065P
85 PS0085P
95 PS0095P
100 PS0100P
115 PS0115P
125 PS0125P
160 PS0160P
195 PS0195P
200 PS0200P
225 PS0225P
250 PS0250P
300 PS0300P
320 PS0320P
355 PS0355P
370 PS0370P
390 PS0390P
400 PS0400P
530 PS0530P
570 PS0570P
600 PS0600P
1000 PS1000P
1300 PS1300P
1600 PS1600P
1700 PS1700P
1800 PS1800P

Lightly Hydrophilic Polystyrene Microspheres

These microspheres are designed to enhance stability, passive adsorption, and reactivity.

Diameter, nm Catalog Number
195 PS0195H
300 PS0300H
358 PS0358H
408 PS0408H
614 PS0614H
840 PS0840H
934 PS0934H
1020 PS1020H

Omni-Bind? Lipophilic Plain-Surface Microspheres

Our innovative process technology and formulations have led to the development of novel lipophilic (lipid incorporated/coated) microspheres for better binding of proteins. Microspheres have been developed to increase the binding efficiency of various proteins.

  • The lipid incorporated/coated microspheres contour to the conformation of the proteins for better adsorption/binding efficiency and improved visibility of the agglutinates
  • We also make it to your exact specs

Diameter, nm Catalog Number
109 LP0109A
119 LP0119A
160 LP0160E
173 LP0173E
313 LP0313E
394 LP0394E
535 LP0535C

Low-Density Plain-Surface Microspheres

These microspheres are highly uniform, stable, and reproducible. They are designed to reduce settling.

Diameter, nm Catalog Number Density
105 LD0105P3 1.025
179 LD0179P3 1.025
249 LD0249P3 1.025
306 LD0306P3 1.025
414 LD0414P2 1.02
415 LD0415P1 1.01
418 LD0418P3 1.025
559 LD0559P3 1.025
723 LD0723P1 1.01
1060 LD1060P1 1.01
1460 LD1460P2 1.02

Non-polystyrene Plain-surface Microspheres

These microspheres are designed to reduce non-specific binding and are easy to centrifuge.

Diameter, nm Catalog Number
100 NP0100P
198 NP0198P
300 NP0300P
400 NP0400P

High-Density Microspheres

Diameter, nm Catalog Number Density
88 HD0088P4 1.37
95 HD0095C1 1.09
184 HD0184P5 1.53
195 HD0195C1 1.09
198 HD0198P2 1.19
246 HD0246C1 1.09
300 HD0300P2 1.19
304 HD0304C1 1.09
360 HD0360C1 1.09
400 HD0400P2 1.19
405 HD0405C1 1.09

Soap-Free Polystyrene Microspheres

Our pioneering research in microparticle technology has led to the development of distinctive soap-free microspheres with the following unique features:

  • Optimum sulfate groups to stabilize the microparticles
  • Innovative process technology and formulation provide stability and uniformity throughout the polymerization
  • No excess sulfate groups to interfere with passive adsorption
  • No residual monomer
  • Very uniform, stable, and reproducible
  • Fluorescent and Dyed microspheres: Available in selected sizes
  • We also make it to your exact specs

Diameter, nm Catalog Number
100 SF0100P
200 SF0200P
300 SF0300P
355 SF0355P
400 SF0400P
600 SF0600P
1000 SF1000P

Fluorescent / Dyed Polystyrene Microspheres

Standard Colors: Fluorescent Yellow, Blue, Red, Hot Pink, Pink, Violet, Coral, Lavender, and other colors

We can dye any product in this catalog to the color of your choice.

Other Products

SupraMag? Superparamagnetic Microparticles

PolyMicrospheres innovation in immunomagnetic research has led to the development of novel superparamagnetic microparticles with the highest magnetic loading and optimized levels of functional groups for covalent coupling of ligands. These highly attractive superparamagnetic microparticles also provide high surface area. The following products exhibit higher attraction to the magnet than typical 1-4 μM magnetic particles.

  • High emu/g
  • Highest magnetic attraction
  • Fast response to magnet
  • Clear supernatant
  • Easy redispersion

Diameter, μM Functionality Catalog No.
0.2 Amino SM0200N
0.25 Amino SM0250N
0.25 Chloromethyl SM0250CL
0.3 Amino SM0300N
0.3 Chloromethyl SM0300CL
0.3 Sulfhydryl SM0300SH
0.4 Amino SM0400N
0.4 Chloromethyl SM0400CL
0.5 Amino SM0500N
0.5 Chloromethyl SM0500CL
0.6 Amino SM0600N
0.6 Chloromethyl SM0600CL
0.7 Amino SM0700N
0.8 Amino SM0800N
1.0 Amino SM1000N

Large Microbeads

Product Diameter Range, μM Mean Diameter, μM
Polystyrene microbeads

density = 1.05

 ~ 9
75-125
125-210
125-300
210-300
<= 300
300-425
PS-Carboxyl microbeads

density = 1.05

 75-125
75-125 ~ 110
125-210
125-210 ~ 150
125-210 ~ 158
150-250 ~ 200
140-300 ~ 235
140-300 ~ 240
210-300 ~ 240
210-300 ~ 260
PS-Carboxyl microbeads

density = 1.04

 37-300
PS-SpaceLink Carboxyl microbeads

density = 1.05

 75-125 ~ 105
125-210 ~ 155
210-300 ~ 220
300-425 ~ 260
PS-Hydroxyl microbeads

density = 1.04

 75-125
125-210
PS-Gelatin coated microbeads

density = 1.05

 75-125
125-300
300-425
425-700
PS-Low Density microbeads

density = 1.03

 75-125
~ 123
~ 152
~ 166
210-250
250-300
37-300
PS-Low Density microbeads

density = 1.04

 37-300
Chloromethyl microbeads

–CH2Cl

 37-75
75-125
125-212
212-250
250-300
300-425
385-535 ~ 465