水溶性抗荧光淬灭封片剂(含DAPI)|DAPI Fluoromount-G™

水溶性抗荧光淬灭封片剂(含DAPI)|DAPI Fluoromount-G™

产品说明书

FAQ

COA

已发表文献

产品描述

DAPI Fluoromount-G™是一种含有蓝色细胞核荧光探针-DAPI(Em=455 nm)的水溶性封片剂,用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片,可以显著减缓各种常见荧光染料的荧光淬灭现象。本封片剂具有半永久性封固性,可用作长期保存玻片样本的制作。适用于细胞制备物和组织切片的封片,兼容于免疫荧光,免疫细胞化学,免疫组化等实验。

本品已加DAPI,可同时完成核染和封片,使得实验操作更简便。封片剂干燥后形成一层牢固的涂层,光学透明,便于后续荧光观察和拍照。

运输和保存方法

室温运输。室温避光保存,一年有效。

使用方法
1) 根据实验室已有的体系制备细胞离心涂片;
2) 固定和再水化细胞涂片;
3) 从染色最后一步的水溶性缓冲液中取出一片玻片,直接滴加一滴 DAPI Fluoromount-G™至细胞涂片,盖上盖玻片,用无纺纱布轻轻按压盖玻片以去除多余分封片剂和封固盖玻片。
4) 室温下避光静置5 min,使得封片剂晾干后再进行普通荧光显微镜或者共聚焦显微检测。5) 若玻片样本需长久保存,可使用指甲油或者中性树胶(货号:36313ES60)对盖玻片四周进行密封。

注意事项
1)本品含有0.1%的叠氮钠作为防腐剂,吞食和吸入有毒,操作时请做好防护。
2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
3)本产品仅作科研用途!

Q:该产品需要低温贮藏吗?

A:室温避光保存即可。

Q:使用该产品后多久可进行显微检测?

A:一般室温下避光静置 5 min,使得封片剂晾干后,再进行普通荧光显微镜或者共聚焦显微检测。

Q:该产品可使玻片长久保存吗?

A:不可。若玻片样本需长久保存,可使用指甲油或者中性树胶对盖玻片四周进行密封。

Q:该产品可用于免疫荧光、免疫细胞化学、免疫组化实验吗?

A:可以。

Q:该产品可用于活细胞吗?

A:不推荐,对于染色活细胞膜染料和线粒体染料的抗淬灭效果不佳,建议抗荧光淬灭封片剂用于固定透化的细胞或者组织。

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产品描述

DAPI Fluoromount-G™是一种含有蓝色细胞核荧光探针-DAPI(Em=455 nm)的水溶性封片剂,用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片,可以显著减缓各种常见荧光染料的荧光淬灭现象。本封片剂具有半永久性封固性,可用作长期保存玻片样本的制作。适用于细胞制备物和组织切片的封片,兼容于免疫荧光,免疫细胞化学,免疫组化等实验。

本品已加DAPI,可同时完成核染和封片,使得实验操作更简便。封片剂干燥后形成一层牢固的涂层,光学透明,便于后续荧光观察和拍照。

运输和保存方法

室温运输。室温避光保存,一年有效。

使用方法
1) 根据实验室已有的体系制备细胞离心涂片;
2) 固定和再水化细胞涂片;
3) 从染色最后一步的水溶性缓冲液中取出一片玻片,直接滴加一滴 DAPI Fluoromount-G™至细胞涂片,盖上盖玻片,用无纺纱布轻轻按压盖玻片以去除多余分封片剂和封固盖玻片。
4) 室温下避光静置5 min,使得封片剂晾干后再进行普通荧光显微镜或者共聚焦显微检测。5) 若玻片样本需长久保存,可使用指甲油或者中性树胶(货号:36313ES60)对盖玻片四周进行密封。

注意事项
1)本品含有0.1%的叠氮钠作为防腐剂,吞食和吸入有毒,操作时请做好防护。
2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
3)本产品仅作科研用途!

Q:该产品需要低温贮藏吗?

A:室温避光保存即可。

Q:使用该产品后多久可进行显微检测?

A:一般室温下避光静置 5 min,使得封片剂晾干后,再进行普通荧光显微镜或者共聚焦显微检测。

Q:该产品可使玻片长久保存吗?

A:不可。若玻片样本需长久保存,可使用指甲油或者中性树胶对盖玻片四周进行密封。

Q:该产品可用于免疫荧光、免疫细胞化学、免疫组化实验吗?

A:可以。

Q:该产品可用于活细胞吗?

A:不推荐,对于染色活细胞膜染料和线粒体染料的抗淬灭效果不佳,建议抗荧光淬灭封片剂用于固定透化的细胞或者组织。

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