细胞/组织miRNA提取试剂盒|MolPure® Cell/Tissue miRNA Kit

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细胞/组织miRNA提取试剂盒|MolPure® Cell/Tissue miRNA Kit

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MolPure® Cell/Tissue miRNA Kit采用MolPure® RNA Column M1和microRNA Column M1以及新型的溶液体系，适用于从多种新鲜或冻存的动物组织或培养细胞中快速提取总RNA、microRNA和其它各种小RNA。独特的裂解液可迅速裂解细胞并灭活RNA酶，操作简便，30 min即可完成动物组织或细胞（50-100 mg动物组织或5×106~1×107个动物细胞）总RNA的提取纯化工作。试剂盒内的MolPure® RNA Column M1可选择性吸附核酸，不吸附蛋白质、多糖和其它非核酸类物质；microRNA Column M1则能从总RNA中有效富集到15-30 nt左右的小RNA（包括siRNA和miRNA）。DNase I直接在柱上消化gDNA，无DNase残留，提取的总RNA纯度高，可直接用于反转录、荧光定量PCR、二代测序、芯片、Northern-blot、RACE、RNAi等多种分子生物学实验。

 

产品组分

类别

编号

组分名称

19331ES08（5 T）

19331ES50（50 T）

Part I

19331-A

裂解液LB (LB Buffer M1)

5 mL

50 mL

Part II

19331–B

RNA吸附柱M1 (MolPure® RNA Column M1)

5 个

50 个

19331–C

miRNA吸附柱M1 (miRNA Column M1)

5 个

50 个

19331–D

2 mL收集管 (2 mL Collection Tube M1)

10 个

100 个

19331-E

去蛋白液PL* (PL Buffer M1*)

1.2 mL

12 mL

19331-F

漂洗液W* (Wash Buffer M1*)

1.3 mL

13 mL

19331-G

RNase-free H2O

1 mL

5 mL

 

运输和保存方法

Part I组分常温运输，4℃避光保存，Part II组分常温运输，常温保存。

产品有效期12个月。

 

注意事项

1. 避免试剂长时间暴露于空气中产生挥发、氧化、pH值变化，各溶液使用后应及时盖紧盖子。

2. 漂洗液W*添加乙醇后，易形成沉淀，不影响使用。使用时，吸取上清即可。

3. 裂解液LB和去蛋白液PL*含有刺激性物质，为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

4. 本产品仅作科研用途！

 

相关产品

产品名称

货号

规格

MolPure® Cell/Tissue Total RNA Kit  细胞/组织总RNA提取试剂盒

19221ES08/50

5/50 T

MolPure® TRIeasy Plus Total RNA Kit  总RNA提取试剂盒(带离心柱）

19211ES60

100 T

Hifair® III 1st Strand cDNA Synthesis Kit (gDNA digester plus)

11139ES60

100 T

Hifair® II 1st Strand cDNA Synthesis Kit (gDNA digester plus)

11121ES60

100 T

Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix

11184ES08

5×1 mL

Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，No Rox)

11195ES08 

5×1 mL

Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，Low Rox)

11196ES08

5×1 mL

Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，High Rox)

11197ES08

5×1 mL

Dual Luciferase Reporter Gene Assay Kit 双荧光素酶报告基因检测试剂盒

11402ES60/80

100/1000 T

 

HB210708

 

细胞/组织miRNA提取试剂盒|MolPure® Cell/Tissue miRNA Kit

暂无内容

[1] Dong Z, Zheng N, Hu C, et al. Nosema bombycis microRNA-like RNA 8 (Nb-milR8) Increases Fungal Pathogenicity by Modulating BmPEX16 Gene Expression in Its Host, Bombyx mori. Microbiol Spectr. 2021;9(2):e0104821. doi:10.1128/Spectrum.01048-21(IF:7.171)
[2] Hu C, Dong Z, Deng B, et al. MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori. J Fungi (Basel). 2021;7(12):1051. Published 2021 Dec 8. doi:10.3390/jof7121051(IF:5.816)
[3] Teng JW, Bian SS, Kong P, Chen YG. Icariin triggers osteogenic differentiation of bone marrow stem cells by up-regulating miR-335-5p. Exp Cell Res. 2022;414(2):113085. doi:10.1016/j.yexcr.2022.113085(IF:3.905)
[4] Cui Z, Li Y, Liu G, Jiang Y. miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis. Dis Markers. 2022;2022:2602717. Published 2022 Feb 23. doi:10.1155/2022/2602717(IF:3.434)
[5] Yi L, Liu J, Deng M, Zuo H, Li M. Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis. BMC Pulm Med. 2021;21(1):252. Published 2021 Jul 31. doi:10.1186/s12890-021-01616-1(IF:3.317)
[6] Liu D, Wan L, Gong H, Chen S, Kong Y, Xiao B. Sevoflurane promotes the apoptosis of laryngeal squamous cell carcinoma in-vitro and inhibits its malignant progression via miR-26a/FOXO1 axis. Bioengineered. 2021;12(1):6364-6376. doi:10.1080/21655979.2021.1962684(IF:3.269)
[7] Shi M, Chen X, Li H, Zheng L. δ-tocotrienol suppresses the migration and angiogenesis of trophoblasts in preeclampsia and promotes their apoptosis via miR-429/ ZEB1 axis. Bioengineered. 2021;12(1):1861-1873. doi:10.1080/21655979.2021.1923238(IF:3.269)
[8] Bi X, Jiang Z, Luan Z, Qiu D. Crocin exerts anti-proliferative and apoptotic effects on cutaneous squamous cell carcinoma via miR-320a/ATG2B. Bioengineered. 2021;12(1):4569-4580. doi:10.1080/21655979.2021.1955175(IF:3.269)
[9] Hu Y, Wu L, Jiang L, et al. Notoginsenoside R2 reduces Aβ25-35-induced neuronal apoptosis and inflammation via miR-27a/SOX8/β-catenin axis. Hum Exp Toxicol. 2021;40(12_suppl):S347-S358. doi:10.1177/09603271211041996(IF:2.903)
[10] Li Y, Qin G, Du J, Yue P, Zhang Y, Hou N. circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway. J Healthc Eng. 2022;2022:2373580. Published 2022 Jan 6. doi:10.1155/2022/2373580(IF:2.682)
[11] Sun Y, Lu X, Li H, Li X. Dihydroartemisinin inhibits IL-6-induced epithelial-mesenchymal transition in laryngeal squamous cell carcinoma via the miR-130b-3p/STAT3/β-catenin signaling pathway. J Int Med Res. 2021;49(11):3000605211009494. doi:10.1177/03000605211009494(IF:1.671)
[12] Guo X, Ji Q, Wu M, Ma W. Naringin attenuates acute myocardial ischemia-reperfusion injury via miR- 126/GSK-3β/β-catenin signaling pathway. Acta Cir Bras. 2022;37(1):e370102. Published 2022 Apr 8. doi:10.1590/acb370102(IF:1.388)

MolPure® Cell/Tissue miRNA Kit采用MolPure® RNA Column M1和microRNA Column M1以及新型的溶液体系，适用于从多种新鲜或冻存的动物组织或培养细胞中快速提取总RNA、microRNA和其它各种小RNA。独特的裂解液可迅速裂解细胞并灭活RNA酶，操作简便，30 min即可完成动物组织或细胞（50-100 mg动物组织或5×106~1×107个动物细胞）总RNA的提取纯化工作。试剂盒内的MolPure® RNA Column M1可选择性吸附核酸，不吸附蛋白质、多糖和其它非核酸类物质；microRNA Column M1则能从总RNA中有效富集到15-30 nt左右的小RNA（包括siRNA和miRNA）。DNase I直接在柱上消化gDNA，无DNase残留，提取的总RNA纯度高，可直接用于反转录、荧光定量PCR、二代测序、芯片、Northern-blot、RACE、RNAi等多种分子生物学实验。

 

产品组分

类别

编号

组分名称

19331ES08（5 T）

19331ES50（50 T）

Part I

19331-A

裂解液LB (LB Buffer M1)

5 mL

50 mL

Part II

19331–B

RNA吸附柱M1 (MolPure® RNA Column M1)

5 个

50 个

19331–C

miRNA吸附柱M1 (miRNA Column M1)

5 个

50 个

19331–D

2 mL收集管 (2 mL Collection Tube M1)

10 个

100 个

19331-E

去蛋白液PL* (PL Buffer M1*)

1.2 mL

12 mL

19331-F

漂洗液W* (Wash Buffer M1*)

1.3 mL

13 mL

19331-G

RNase-free H2O

1 mL

5 mL

 

运输和保存方法

Part I组分常温运输，4℃避光保存，Part II组分常温运输，常温保存。

产品有效期12个月。

 

注意事项

1. 避免试剂长时间暴露于空气中产生挥发、氧化、pH值变化，各溶液使用后应及时盖紧盖子。

2. 漂洗液W*添加乙醇后，易形成沉淀，不影响使用。使用时，吸取上清即可。

3. 裂解液LB和去蛋白液PL*含有刺激性物质，为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

4. 本产品仅作科研用途！

 

相关产品

产品名称

货号

规格

MolPure® Cell/Tissue Total RNA Kit  细胞/组织总RNA提取试剂盒

19221ES08/50

5/50 T

MolPure® TRIeasy Plus Total RNA Kit  总RNA提取试剂盒(带离心柱）

19211ES60

100 T

Hifair® III 1st Strand cDNA Synthesis Kit (gDNA digester plus)

11139ES60

100 T

Hifair® II 1st Strand cDNA Synthesis Kit (gDNA digester plus)

11121ES60

100 T

Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix

11184ES08

5×1 mL

Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，No Rox)

11195ES08 

5×1 mL

Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，Low Rox)

11196ES08

5×1 mL

Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，High Rox)

11197ES08

5×1 mL

Dual Luciferase Reporter Gene Assay Kit 双荧光素酶报告基因检测试剂盒

11402ES60/80

100/1000 T

 

HB210708

 

细胞/组织miRNA提取试剂盒|MolPure® Cell/Tissue miRNA Kit

暂无内容

[1] Dong Z, Zheng N, Hu C, et al. Nosema bombycis microRNA-like RNA 8 (Nb-milR8) Increases Fungal Pathogenicity by Modulating BmPEX16 Gene Expression in Its Host, Bombyx mori. Microbiol Spectr. 2021;9(2):e0104821. doi:10.1128/Spectrum.01048-21(IF:7.171)
[2] Hu C, Dong Z, Deng B, et al. MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori. J Fungi (Basel). 2021;7(12):1051. Published 2021 Dec 8. doi:10.3390/jof7121051(IF:5.816)
[3] Teng JW, Bian SS, Kong P, Chen YG. Icariin triggers osteogenic differentiation of bone marrow stem cells by up-regulating miR-335-5p. Exp Cell Res. 2022;414(2):113085. doi:10.1016/j.yexcr.2022.113085(IF:3.905)
[4] Cui Z, Li Y, Liu G, Jiang Y. miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis. Dis Markers. 2022;2022:2602717. Published 2022 Feb 23. doi:10.1155/2022/2602717(IF:3.434)
[5] Yi L, Liu J, Deng M, Zuo H, Li M. Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis. BMC Pulm Med. 2021;21(1):252. Published 2021 Jul 31. doi:10.1186/s12890-021-01616-1(IF:3.317)
[6] Liu D, Wan L, Gong H, Chen S, Kong Y, Xiao B. Sevoflurane promotes the apoptosis of laryngeal squamous cell carcinoma in-vitro and inhibits its malignant progression via miR-26a/FOXO1 axis. Bioengineered. 2021;12(1):6364-6376. doi:10.1080/21655979.2021.1962684(IF:3.269)
[7] Shi M, Chen X, Li H, Zheng L. δ-tocotrienol suppresses the migration and angiogenesis of trophoblasts in preeclampsia and promotes their apoptosis via miR-429/ ZEB1 axis. Bioengineered. 2021;12(1):1861-1873. doi:10.1080/21655979.2021.1923238(IF:3.269)
[8] Bi X, Jiang Z, Luan Z, Qiu D. Crocin exerts anti-proliferative and apoptotic effects on cutaneous squamous cell carcinoma via miR-320a/ATG2B. Bioengineered. 2021;12(1):4569-4580. doi:10.1080/21655979.2021.1955175(IF:3.269)
[9] Hu Y, Wu L, Jiang L, et al. Notoginsenoside R2 reduces Aβ25-35-induced neuronal apoptosis and inflammation via miR-27a/SOX8/β-catenin axis. Hum Exp Toxicol. 2021;40(12_suppl):S347-S358. doi:10.1177/09603271211041996(IF:2.903)
[10] Li Y, Qin G, Du J, Yue P, Zhang Y, Hou N. circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway. J Healthc Eng. 2022;2022:2373580. Published 2022 Jan 6. doi:10.1155/2022/2373580(IF:2.682)
[11] Sun Y, Lu X, Li H, Li X. Dihydroartemisinin inhibits IL-6-induced epithelial-mesenchymal transition in laryngeal squamous cell carcinoma via the miR-130b-3p/STAT3/β-catenin signaling pathway. J Int Med Res. 2021;49(11):3000605211009494. doi:10.1177/03000605211009494(IF:1.671)
[12] Guo X, Ji Q, Wu M, Ma W. Naringin attenuates acute myocardial ischemia-reperfusion injury via miR- 126/GSK-3β/β-catenin signaling pathway. Acta Cir Bras. 2022;37(1):e370102. Published 2022 Apr 8. doi:10.1590/acb370102(IF:1.388)