小鼠抗Flag-Tag单克隆抗体 DYKDDDDK|Flag-Tag Mouse mAb(DYKDDDDK)

发表于由

小鼠抗Flag-Tag单克隆抗体 DYKDDDDK|Flag-Tag Mouse mAb(DYKDDDDK)

产品说明书

FAQ

COA

已发表文献

产品描述

 

标签抗原由于其分子量小，对融合蛋白的活性没有影响等特点，已被广泛应用于重组蛋白在后续免疫印迹、免疫沉淀和免疫技术的检测中。Flag标签是重组蛋白研究中应用较为广泛的一种，该标签系统利用一个短的亲水性八氨基酸肽（DYKDDDDK）融合到目标蛋白的N-端或者C-端，且该标签可被后续肠激酶去除。Flag抗体可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位，以及纯化、定性或定量检测Flag融合表达蛋白等。

 

产品性质

 

别名

DDDDK;DDDDK tag;DDDDK-tag

免疫原

Synthetic Flag tag peptide coupled to KLH

缓冲液

50% Glycerol, 150mM Sodium Chloride, 10mM Sodium Phosphate, pH 7.4

克隆类型 

Monoclonal antibody

亚型

IgG2b

宿主

Mouse

推荐应用

WB,IF,IP,ELISA

物种反应

Transfected

纯化方式

Affinity Chromatography

浓度

见管壁

 

应用

 

推荐稀释比：WB1:1000-1:5000;IF：1:1000;IP：1:100;ELISA：1:2000

 

运输与保存方法

 

冰袋运输，-20℃保存，有效期一年。

 

注意事项

 

避免反复冻融。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

本产品仅作科研用途！

HB221114

小鼠抗Flag-Tag单克隆抗体 DYKDDDDK|Flag-Tag Mouse mAb(DYKDDDDK)

暂无内容

[1] Dong W, Zhu Y, Chang H, et al. An SHR-SCR module specifies legume cortical cell fate to enable nodulation. Nature. 2021;589(7843):586-590. doi:10.1038/s41586-020-3016-z(IF:42.779)
[2] Song Y, Guo Y, Li X, et al. RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)
[3] Liu M, Zhang Y, Wu Y, et al. IKZF1 selectively enhances homologous recombination repair by interacting with CtIP and USP7 in multiple myeloma. Int J Biol Sci. 2022;18(6):2515-2526. Published 2022 Mar 21. doi:10.7150/ijbs.70960(IF:6.582)
[4] Wu Q, Huang Y, Gu L, Chang Z, Li GM. OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. J Biol Chem. 2021;296:100466. doi:10.1016/j.jbc.2021.100466(IF:5.157)
[5] Sun R, Guo Y, Li X, et al. PRRSV Non-Structural Proteins Orchestrate Porcine E3 Ubiquitin Ligase RNF122 to Promote PRRSV Proliferation. Viruses. 2022;14(2):424. Published 2022 Feb 18. doi:10.3390/v14020424(IF:5.048)
[6] Chen N, Chen G, Kong X, Wu X. Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin (polh) and p10 as a Component of Transcription Initiation Complex (TIC). Viruses. 2022;14(1):153. Published 2022 Jan 14. doi:10.3390/v14010153(IF:5.048)
[7] Wang D, Tan KS, Zeng W, et al. Hepatocellular BChE as a therapeutic target to ameliorate hypercholesterolemia through PRMT5 selective degradation to restore LDL receptor transcription. Life Sci. 2022;293:120336. doi:10.1016/j.lfs.2022.120336(IF:5.037)
[8] Chen G, Kong Y, Li Y, et al. A Promising Intracellular Protein-Degradation Strategy: TRIMbody-Away Technique Based on Nanobody Fragment. Biomolecules. 2021;11(10):1512. Published 2021 Oct 14. doi:10.3390/biom11101512(IF:4.879)
[9] Tan KS, Zhang Y, Liu L, et al. Molecular cloning and characterization of an atypical butyrylcholinesterase-like protein in zebrafish. Comp Biochem Physiol B Biochem Mol Biol. 2021;255:110590. doi:10.1016/j.cbpb.2021.110590(IF:2.231)

产品描述

 

标签抗原由于其分子量小，对融合蛋白的活性没有影响等特点，已被广泛应用于重组蛋白在后续免疫印迹、免疫沉淀和免疫技术的检测中。Flag标签是重组蛋白研究中应用较为广泛的一种，该标签系统利用一个短的亲水性八氨基酸肽（DYKDDDDK）融合到目标蛋白的N-端或者C-端，且该标签可被后续肠激酶去除。Flag抗体可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位，以及纯化、定性或定量检测Flag融合表达蛋白等。

 

产品性质

 

别名

DDDDK;DDDDK tag;DDDDK-tag

免疫原

Synthetic Flag tag peptide coupled to KLH

缓冲液

50% Glycerol, 150mM Sodium Chloride, 10mM Sodium Phosphate, pH 7.4

克隆类型 

Monoclonal antibody

亚型

IgG2b

宿主

Mouse

推荐应用

WB,IF,IP,ELISA

物种反应

Transfected

纯化方式

Affinity Chromatography

浓度

见管壁

 

应用

 

推荐稀释比：WB1:1000-1:5000;IF：1:1000;IP：1:100;ELISA：1:2000

 

运输与保存方法

 

冰袋运输，-20℃保存，有效期一年。

 

注意事项

 

避免反复冻融。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

本产品仅作科研用途！

HB221114

小鼠抗Flag-Tag单克隆抗体 DYKDDDDK|Flag-Tag Mouse mAb(DYKDDDDK)

暂无内容

[1] Dong W, Zhu Y, Chang H, et al. An SHR-SCR module specifies legume cortical cell fate to enable nodulation. Nature. 2021;589(7843):586-590. doi:10.1038/s41586-020-3016-z(IF:42.779)
[2] Song Y, Guo Y, Li X, et al. RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)
[3] Liu M, Zhang Y, Wu Y, et al. IKZF1 selectively enhances homologous recombination repair by interacting with CtIP and USP7 in multiple myeloma. Int J Biol Sci. 2022;18(6):2515-2526. Published 2022 Mar 21. doi:10.7150/ijbs.70960(IF:6.582)
[4] Wu Q, Huang Y, Gu L, Chang Z, Li GM. OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. J Biol Chem. 2021;296:100466. doi:10.1016/j.jbc.2021.100466(IF:5.157)
[5] Sun R, Guo Y, Li X, et al. PRRSV Non-Structural Proteins Orchestrate Porcine E3 Ubiquitin Ligase RNF122 to Promote PRRSV Proliferation. Viruses. 2022;14(2):424. Published 2022 Feb 18. doi:10.3390/v14020424(IF:5.048)
[6] Chen N, Chen G, Kong X, Wu X. Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin (polh) and p10 as a Component of Transcription Initiation Complex (TIC). Viruses. 2022;14(1):153. Published 2022 Jan 14. doi:10.3390/v14010153(IF:5.048)
[7] Wang D, Tan KS, Zeng W, et al. Hepatocellular BChE as a therapeutic target to ameliorate hypercholesterolemia through PRMT5 selective degradation to restore LDL receptor transcription. Life Sci. 2022;293:120336. doi:10.1016/j.lfs.2022.120336(IF:5.037)
[8] Chen G, Kong Y, Li Y, et al. A Promising Intracellular Protein-Degradation Strategy: TRIMbody-Away Technique Based on Nanobody Fragment. Biomolecules. 2021;11(10):1512. Published 2021 Oct 14. doi:10.3390/biom11101512(IF:4.879)
[9] Tan KS, Zhang Y, Liu L, et al. Molecular cloning and characterization of an atypical butyrylcholinesterase-like protein in zebrafish. Comp Biochem Physiol B Biochem Mol Biol. 2021;255:110590. doi:10.1016/j.cbpb.2021.110590(IF:2.231)