布雷非德菌素A 蛋白转运抑制剂|Brefeldin A(BFA,5 mg/mL)|CAS 20350-15-6

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布雷非德菌素A 蛋白转运抑制剂|Brefeldin A(BFA,5 mg/mL)|CAS 20350-15-6

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已发表文献

产品描述

Brefeldin A，简称BFA，一种常用的可逆性蛋白转运抑制剂，是一种真菌代谢产物，可破坏高尔基体的结构并抑制其功能，进而特异地阻断蛋白质从内质网(ER)向高尔基体(Golgi)的转运。BFA 可抑制coat proteins（如β-COP and ADP-ribosylation factor (ARF)）与高尔基体的结合并抑制GDP-GTP交换。

Brefeldin A也常被用于抑制细胞因子等蛋白的分泌，并用于增强分泌蛋白的免疫染色检测。Brefeldin A可激活神经鞘磷脂循环(sphingomyelin cycle)，还可诱导一些肿瘤细胞发生凋亡。

本品为溶于DMSO的BFA储存液，浓度为5 mg/mL，推荐使用浓度范围是1-50 μM，具体的最佳工作浓度请参考相关文献，并根据实验目的以及所用特定细胞和组织，进行优化。

 

产品性质

英文别名 (English Synonym)

γ,4-Dihydroxy-2-[6-hydroxy-1-heptenyl]-4-cyclopentanecrotonic acid λ-lactone; cyanein; BFA；Decumbin

中文名称 (Chinese Name)

布雷非德菌素A

通路 (Pathway)

Autophagy–Autophagy

CAS号 (CAS NO.)

20350-15-6

分子式 (Formula)

C16H24O4

分子量 (Molecular Weight)

280.36

纯度 (Purity)

≥99%

溶解性 (Solubility)

溶于DMSO (100 mg/mL)

结构式 (Structure)

布雷非德菌素A 蛋白转运抑制剂|Brefeldin A(BFA,5 mg/mL)|CAS 20350-15-6 

 

运输和保存方法

冰袋运输。溶液在-80℃稳定保存2年，-20℃稳定保存1年，4℃稳定保存6个月。

 

注意事项

1. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心，以保证产品全在管底。

3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。

4. 本产品仅用于科研用途，禁止用于人身上。

 

客户使用本产品发表的科研文献（部分）

[1] Wang C, Zhang S, Liu J, et al. Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway. Cell Rep. 2020 Feb 11;30(6):1780-1797.e6. IF: 8.109

[2] Zhang S, Wang C, Ma B, et al. Mutant p53 Drives Cancer Metastasis via RCP-Mediated Hsp90α Secretion. Cell Rep. 2020 Jul 7;32(1):107879. IF: 8.109

[3]  Lin DL, Yao HY, Jia LH, et al. Phospholipase D-derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN-FORMED2. New Phytol. 2020 Apr;226(1):142-155. IF: 7.299

[4] Ma H, Tan Y, Wen D, et al. DC-CTL targeting carbonic anhydrase IX gene combined with iAPA therapy in the treatment of renal cell carcinoma. Hum Vaccin Immunother. 2021 Nov 2;17(11):4363-4373. IF: 3.452

[5] Zhao M, Duan N, Wang Y, et al. CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome[J]. Autoimmunity. 2019 Feb;52(1):12-20. IF:2.403

[6] Shen ZY, Wu B, Liu T, et al. Immunomodulatory effects of bone marrow mesenchymal stem cells overexpressing heme oxygenase-1: Protective effects on acute rejection following reduced-size liver transplantation in a rat model. Cell Immunol. 2017 Mar;313:10-24. IF: 2.399

 

HB220909

 

布雷非德菌素A 蛋白转运抑制剂|Brefeldin A(BFA,5 mg/mL)|CAS 20350-15-6

暂无内容

[1] Wang C, Zhang S, Liu J, et al. Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway. Cell Rep. 2020;30(6):1780-1797.e6. doi:10.1016/j.celrep.2020.01.037(IF:8.109)
[2] Zhang S, Wang C, Ma B, et al. Mutant p53 Drives Cancer Metastasis via RCP-Mediated Hsp90α Secretion. Cell Rep. 2020;32(1):107879. doi:10.1016/j.celrep.2020.107879(IF:8.109)
[3] Lin DL, Yao HY, Jia LH, et al. Phospholipase D-derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN-FORMED2. New Phytol. 2020;226(1):142-155. doi:10.1111/nph.16330(IF:7.299)
[4] Ma H, Tan Y, Wen D, et al. DC-CTL targeting carbonic anhydrase IX gene combined with iAPA therapy in the treatment of renal cell carcinoma. Hum Vaccin Immunother. 2021;17(11):4363-4373. doi:10.1080/21645515.2021.1955610(IF:3.452)
[5] Zhao M, Duan N, Wang Y, et al. CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome. Autoimmunity. 2019;52(1):12-20. doi:10.1080/08916934.2019.1576644(IF:2.403)
[6] Shen ZY, Wu B, Liu T, et al. Immunomodulatory effects of bone marrow mesenchymal stem cells overexpressing heme oxygenase-1: Protective effects on acute rejection following reduced-size liver transplantation in a rat model. Cell Immunol. 2017;313:10-24. doi:10.1016/j.cellimm.2016.12.006(IF:2.399)

产品描述

Brefeldin A，简称BFA，一种常用的可逆性蛋白转运抑制剂，是一种真菌代谢产物，可破坏高尔基体的结构并抑制其功能，进而特异地阻断蛋白质从内质网(ER)向高尔基体(Golgi)的转运。BFA 可抑制coat proteins（如β-COP and ADP-ribosylation factor (ARF)）与高尔基体的结合并抑制GDP-GTP交换。

Brefeldin A也常被用于抑制细胞因子等蛋白的分泌，并用于增强分泌蛋白的免疫染色检测。Brefeldin A可激活神经鞘磷脂循环(sphingomyelin cycle)，还可诱导一些肿瘤细胞发生凋亡。

本品为溶于DMSO的BFA储存液，浓度为5 mg/mL，推荐使用浓度范围是1-50 μM，具体的最佳工作浓度请参考相关文献，并根据实验目的以及所用特定细胞和组织，进行优化。

 

产品性质

英文别名 (English Synonym)

γ,4-Dihydroxy-2-[6-hydroxy-1-heptenyl]-4-cyclopentanecrotonic acid λ-lactone; cyanein; BFA；Decumbin

中文名称 (Chinese Name)

布雷非德菌素A

通路 (Pathway)

Autophagy–Autophagy

CAS号 (CAS NO.)

20350-15-6

分子式 (Formula)

C16H24O4

分子量 (Molecular Weight)

280.36

纯度 (Purity)

≥99%

溶解性 (Solubility)

溶于DMSO (100 mg/mL)

结构式 (Structure)

布雷非德菌素A 蛋白转运抑制剂|Brefeldin A(BFA,5 mg/mL)|CAS 20350-15-6 

 

运输和保存方法

冰袋运输。溶液在-80℃稳定保存2年，-20℃稳定保存1年，4℃稳定保存6个月。

 

注意事项

1. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心，以保证产品全在管底。

3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。

4. 本产品仅用于科研用途，禁止用于人身上。

 

客户使用本产品发表的科研文献（部分）

[1] Wang C, Zhang S, Liu J, et al. Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway. Cell Rep. 2020 Feb 11;30(6):1780-1797.e6. IF: 8.109

[2] Zhang S, Wang C, Ma B, et al. Mutant p53 Drives Cancer Metastasis via RCP-Mediated Hsp90α Secretion. Cell Rep. 2020 Jul 7;32(1):107879. IF: 8.109

[3]  Lin DL, Yao HY, Jia LH, et al. Phospholipase D-derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN-FORMED2. New Phytol. 2020 Apr;226(1):142-155. IF: 7.299

[4] Ma H, Tan Y, Wen D, et al. DC-CTL targeting carbonic anhydrase IX gene combined with iAPA therapy in the treatment of renal cell carcinoma. Hum Vaccin Immunother. 2021 Nov 2;17(11):4363-4373. IF: 3.452

[5] Zhao M, Duan N, Wang Y, et al. CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome[J]. Autoimmunity. 2019 Feb;52(1):12-20. IF:2.403

[6] Shen ZY, Wu B, Liu T, et al. Immunomodulatory effects of bone marrow mesenchymal stem cells overexpressing heme oxygenase-1: Protective effects on acute rejection following reduced-size liver transplantation in a rat model. Cell Immunol. 2017 Mar;313:10-24. IF: 2.399

 

HB220909

 

布雷非德菌素A 蛋白转运抑制剂|Brefeldin A(BFA,5 mg/mL)|CAS 20350-15-6

暂无内容

[1] Wang C, Zhang S, Liu J, et al. Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway. Cell Rep. 2020;30(6):1780-1797.e6. doi:10.1016/j.celrep.2020.01.037(IF:8.109)
[2] Zhang S, Wang C, Ma B, et al. Mutant p53 Drives Cancer Metastasis via RCP-Mediated Hsp90α Secretion. Cell Rep. 2020;32(1):107879. doi:10.1016/j.celrep.2020.107879(IF:8.109)
[3] Lin DL, Yao HY, Jia LH, et al. Phospholipase D-derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN-FORMED2. New Phytol. 2020;226(1):142-155. doi:10.1111/nph.16330(IF:7.299)
[4] Ma H, Tan Y, Wen D, et al. DC-CTL targeting carbonic anhydrase IX gene combined with iAPA therapy in the treatment of renal cell carcinoma. Hum Vaccin Immunother. 2021;17(11):4363-4373. doi:10.1080/21645515.2021.1955610(IF:3.452)
[5] Zhao M, Duan N, Wang Y, et al. CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome. Autoimmunity. 2019;52(1):12-20. doi:10.1080/08916934.2019.1576644(IF:2.403)
[6] Shen ZY, Wu B, Liu T, et al. Immunomodulatory effects of bone marrow mesenchymal stem cells overexpressing heme oxygenase-1: Protective effects on acute rejection following reduced-size liver transplantation in a rat model. Cell Immunol. 2017;313:10-24. doi:10.1016/j.cellimm.2016.12.006(IF:2.399)