蛋白A/G琼脂糖纯化树脂 蛋白A/G纯化树脂|rProtein A/G Agarose Resin
产品说明书
FAQ
COA
已发表文献
产品简介
天然蛋白A(Protein A)是一种发现于金黄色葡萄球菌的细胞壁表面蛋白,天然蛋白G(Protein G)是一种分离自G型或C型链球菌属的细胞表面蛋白,二者功能相似,主要通过与免疫球蛋白(Ig)的Fc区相互作用,可结合大多数哺乳动物的IgG。然而两者结合特异性上有所不同(详细见附录1,蛋白A,G对不同物种Ig的结合能力总表)。蛋白A,蛋白G常共价偶联在固体载体如琼脂糖珠或丙烯酸树脂小珠上,直接用以做免疫沉淀(IP)或者免疫共沉淀(Co-IP)来进行蛋白功能相关研究,或者直接装在层析柱上来纯化单克隆或者多克隆抗体。
本品使用的是基因改造后的蛋白A和蛋白G,不仅维持其本身的Ig亲和特性,同时也去除了天然蛋白本身的非主要结合域以降低非特异性结合。本品同时将蛋白A和蛋白G共价偶联到琼脂糖凝胶微球表面,比单独的蛋白A或者蛋白G都有更广的结合范围,适合于所有蛋白A琼脂糖珠和蛋白G琼脂糖珠单独可以结合的Ig,实用性更高。
产品信息
货号 |
36403ES03 / 36403ES05/36403ES08/36403ES25/36403ES60 |
规格 |
1 mL /2 mL/5 mL/25 mL/100 mL |
产品性质
基质(Matrix) |
高度交联的4%琼脂糖微球 |
配体(Ligand) |
重组蛋白A/G |
孔径(Bead size) |
45-165 µm |
最大流速(Flowmax) |
0.3 MPa, 3 bar |
储存缓冲液(Buffer) |
含20%乙醇的1×PBS |
载量(Capacity) |
约10-15 mg Rabbit IgG/mL 基质 |
pH范围(pH range) |
3-10 |
储存条件
2~8℃保存,有效期2年。
附录1. 蛋白A,G对不同物种Ig的结合能力总表
免疫球蛋白亚型 |
Protein A |
Protein G |
免疫球蛋白亚型 |
Protein A |
Protein G |
Human IgG |
++++ |
++++ |
Mouse IgG |
++++ |
++++ |
Human IgG1 |
++++ |
++++ |
Mouse IgG1 |
+ |
++++ |
Human IgG2 |
++++ |
++++ |
Mouse IgG2a |
++++ |
++++ |
Human IgG3 |
+ |
++++ |
Mouse IgG2b |
+++ |
+++ |
Human IgG4 |
++++ |
++++ |
Mouse IgG3 |
++ |
+++ |
Human IgM |
Use anti-Human IgM |
Mouse IgM |
Use anti-Mouse IgM |
||
Human IgE |
NR |
NR |
Chicken IgG (IgY) |
NR |
NR |
Human IgA |
+ |
NR |
Cow IgG |
++ |
++++ |
Human IgA1 |
+ |
NR |
Goat IgG |
+ |
++++ |
Human IgA2 |
+ |
NR |
Goat IgG1 |
+ |
++++ |
Human IgD |
Use anti-Human IgD |
Goat IgG2 |
++++ |
++++ |
|
Rat IgG |
+ |
++ |
Goat IgM |
NR |
NR |
Rat IgG1 |
NR |
+ |
Guinea Pig IgG |
++++ |
++ |
Rat IgG2a |
NR |
NR |
Guinea Pig IgG1 |
++++ |
++ |
Rat IgG2b |
NR |
+ |
Guinea Pig IgG2 |
++++ |
++ |
Rat IgG3 |
+ |
++ |
Hamster IgG |
+ |
++ |
Sheep IgG |
+ |
++ |
Horse IgG |
+ |
++++ |
Sheep IgG1 |
+ |
++ |
Rabbit IgG |
++++ |
+++ |
Sheep IgG2 |
+ |
++ |
Rabbit IgM |
NR |
NR |
Sheep IgM |
NR |
NR |
Rabbit All isotypes |
+++ |
++ |
Pig IgG |
+++ |
+++ |
Monkey IgG |
++++ |
++++ |
Cat IgG |
++++ |
+ |
Donkey IgG |
++ |
++++ |
Dog IgG |
++++ |
+ |
|
|
|
(+)= weak binding;(++)= moderate binding;(++++)= strong binding;NR= not recommended; (-)= not tested; |
Ver.CN20230824
产品简介
天然蛋白A(Protein A)是一种发现于金黄色葡萄球菌的细胞壁表面蛋白,天然蛋白G(Protein G)是一种分离自G型或C型链球菌属的细胞表面蛋白,二者功能相似,主要通过与免疫球蛋白(Ig)的Fc区相互作用,可结合大多数哺乳动物的IgG。然而两者结合特异性上有所不同(详细见附录1,蛋白A,G对不同物种Ig的结合能力总表)。蛋白A,蛋白G常共价偶联在固体载体如琼脂糖珠或丙烯酸树脂小珠上,直接用以做免疫沉淀(IP)或者免疫共沉淀(Co-IP)来进行蛋白功能相关研究,或者直接装在层析柱上来纯化单克隆或者多克隆抗体。
本品使用的是基因改造后的蛋白A和蛋白G,不仅维持其本身的Ig亲和特性,同时也去除了天然蛋白本身的非主要结合域以降低非特异性结合。本品同时将蛋白A和蛋白G共价偶联到琼脂糖凝胶微球表面,比单独的蛋白A或者蛋白G都有更广的结合范围,适合于所有蛋白A琼脂糖珠和蛋白G琼脂糖珠单独可以结合的Ig,实用性更高。
产品信息
货号 |
36403ES03 / 36403ES05/36403ES08/36403ES25/36403ES60 |
规格 |
1 mL /2 mL/5 mL/25 mL/100 mL |
产品性质
基质(Matrix) |
高度交联的4%琼脂糖微球 |
配体(Ligand) |
重组蛋白A/G |
孔径(Bead size) |
45-165 µm |
最大流速(Flowmax) |
0.3 MPa, 3 bar |
储存缓冲液(Buffer) |
含20%乙醇的1×PBS |
载量(Capacity) |
约10-15 mg Rabbit IgG/mL 基质 |
pH范围(pH range) |
3-10 |
储存条件
2~8℃保存,有效期2年。
附录1. 蛋白A,G对不同物种Ig的结合能力总表
免疫球蛋白亚型 |
Protein A |
Protein G |
免疫球蛋白亚型 |
Protein A |
Protein G |
Human IgG |
++++ |
++++ |
Mouse IgG |
++++ |
++++ |
Human IgG1 |
++++ |
++++ |
Mouse IgG1 |
+ |
++++ |
Human IgG2 |
++++ |
++++ |
Mouse IgG2a |
++++ |
++++ |
Human IgG3 |
+ |
++++ |
Mouse IgG2b |
+++ |
+++ |
Human IgG4 |
++++ |
++++ |
Mouse IgG3 |
++ |
+++ |
Human IgM |
Use anti-Human IgM |
Mouse IgM |
Use anti-Mouse IgM |
||
Human IgE |
NR |
NR |
Chicken IgG (IgY) |
NR |
NR |
Human IgA |
+ |
NR |
Cow IgG |
++ |
++++ |
Human IgA1 |
+ |
NR |
Goat IgG |
+ |
++++ |
Human IgA2 |
+ |
NR |
Goat IgG1 |
+ |
++++ |
Human IgD |
Use anti-Human IgD |
Goat IgG2 |
++++ |
++++ |
|
Rat IgG |
+ |
++ |
Goat IgM |
NR |
NR |
Rat IgG1 |
NR |
+ |
Guinea Pig IgG |
++++ |
++ |
Rat IgG2a |
NR |
NR |
Guinea Pig IgG1 |
++++ |
++ |
Rat IgG2b |
NR |
+ |
Guinea Pig IgG2 |
++++ |
++ |
Rat IgG3 |
+ |
++ |
Hamster IgG |
+ |
++ |
Sheep IgG |
+ |
++ |
Horse IgG |
+ |
++++ |
Sheep IgG1 |
+ |
++ |
Rabbit IgG |
++++ |
+++ |
Sheep IgG2 |
+ |
++ |
Rabbit IgM |
NR |
NR |
Sheep IgM |
NR |
NR |
Rabbit All isotypes |
+++ |
++ |
Pig IgG |
+++ |
+++ |
Monkey IgG |
++++ |
++++ |
Cat IgG |
++++ |
+ |
Donkey IgG |
++ |
++++ |
Dog IgG |
++++ |
+ |
|
|
|
(+)= weak binding;(++)= moderate binding;(++++)= strong binding;NR= not recommended; (-)= not tested; |
Ver.CN20230824
Q:为什么柱子反压过高?
A:可能是填料被堵塞;裂解液中含有微笑的固体颗粒,建议上柱前使用 0.22 µm/0.45
µm 滤膜过滤。
Q:为什么洗脱组分中没有目的蛋白?
A:可能是抗体被降解;建议适当的提高洗脱pH。
Q:该产品可以直接使用吗?
A:Protein A 琼脂糖珠最好在使用前充分颠倒若干次,使琼脂糖珠混合均匀。
Q:该产品可以贮存在–20℃吗?
A:不可以,不可冷冻该产品。应置于 4°贮存。
Q:说明书中COIP实验操作中取适量填料是要加多少量的填料?
A:一般填料的量是根据自己要做的样品的量决定。最小的常用COIP体系为:20ul填料+5ug抗体与总体积500ul 总蛋白浓度1mg/ml的裂解液混合孵育。
[1] Chen LJ, Zhang NN, Zhou CX, et al. Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation. Cell Death Differ. 2022;29(2):366-380. doi:10.1038/s41418-021-00861-5(IF:15.828)
[2] Zhang K, Chen S, Yang Q, et al. The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents [published correction appears in Nat Commun. 2022 Jun 1;13(1):3164]. Nat Commun. 2022;13(1):1423. Published 2022 Mar 17. doi:10.1038/s41467-022-29068-z(IF:14.919)
[3] Zhu D, Chen C, Liu X, et al. Osteosarcoma cell proliferation suppression via SHP-2-mediated inactivation of the JAK/STAT3 pathway by tubocapsenolide A. J Adv Res. 2021;34:79-91. Published 2021 Jun 11. doi:10.1016/j.jare.2021.06.004(IF:10.479)
[4] Zheng J, Yao L, Zhou Y, et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021;12(10):871. Published 2021 Sep 24. doi:10.1038/s41419-021-04159-9(IF:8.469)
[5] Wang J, Zhao D, Ding CZ, et al. MicroRNA-194: a novel regulator of glucagon-like peptide-1 synthesis in intestinal L cells. Cell Death Dis. 2021;12(1):113. Published 2021 Jan 21. doi:10.1038/s41419-020-03366-0(IF:8.469)
[6] Zhu H, Guo Y, Huang A, et al. HDAC3-Regulated PGE2 Production by Microglia Induces Phobic Anxiety Susceptibility After Stroke and Pointedly Exploiting a Signal-Targeted Gamma Visual Stimulation New Therapy. Front Immunol. 2022;13:845678. Published 2022 Feb 18. doi:10.3389/fimmu.2022.845678(IF:7.561)
[7] Wang Z, Xu J, Feng J, et al. Structural and Functional Analyses of Type I IFNa Shed Light Into Its Interaction With Multiple Receptors in Fish. Front Immunol. 2022;13:862764. Published 2022 Mar 22. doi:10.3389/fimmu.2022.862764(IF:7.561)
[8] Ni H, Qin H, Sun C, et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis. Stem Cell Res Ther. 2021;12(1):325. Published 2021 Jun 5. doi:10.1186/s13287-021-02394-7(IF:6.832)
[9] Gao L, Guo Q, Li X, et al. MiR-873/PD-L1 axis regulates the stemness of breast cancer cells [published correction appears in EBioMedicine. 2019 Nov;49:389-390]. EBioMedicine. 2019;41:395-407. doi:10.1016/j.ebiom.2019.02.034(IF:6.680)
[10] Yang X, Li X, Zhong C, et al. Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2. Oxid Med Cell Longev. 2021;2021:3823122. Published 2021 Nov 8. doi:10.1155/2021/3823122(IF:6.543)
[11] Xie Y, Peng J, Pang J, et al. Biglycan regulates neuroinflammation by promoting M1 microglial activation in early brain injury after experimental subarachnoid hemorrhage. J Neurochem. 2020;152(3):368-380. doi:10.1111/jnc.14926(IF:4.870)
[12] Sun Z, Zhang L, Li L, et al. Galectin-3 mediates cardiac remodeling caused by impaired glucose and lipid metabolism through inhibiting two pathways of activating Akt. Am J Physiol Heart Circ Physiol. 2021;320(1):H364-H380. doi:10.1152/ajpheart.00523.2020(IF:4.733)
[13] Zhao X, Huang W, Guo J, et al. PLAAT1 promotes p53 degradation via autophagy-lysosome pathway in zebrafish. Fish Shellfish Immunol. 2022;125:48-53. doi:10.1016/j.fsi.2022.05.001(IF:4.581)
[14] Gu H, Duan Z. Silencing of circDPP4 suppresses cell progression of human prostate cancer and enhances docetaxel cytotoxicity through regulating the miR-564/ZIC2 axis. J Gene Med. 2022;24(3):e3403. doi:10.1002/jgm.3403(IF:4.565)
[15] Zheng L, Zhang Z, Zhang S, et al. RNA Binding Protein RNPC1 Inhibits Breast Cancer Cell Metastasis via Activating STARD13-Correlated ceRNA Network. Mol Pharm. 2018;15(6):2123-2132. doi:10.1021/acs.molpharmaceut.7b01123(IF:4.556)
[16] Guo X, Xiang C, Zhang Z, Zhang F, Xi T, Zheng L. Displacement of Bax by BMF Mediates STARD13 3'UTR-Induced Breast Cancer Cells Apoptosis in an miRNA-Depedent Manner. Mol Pharm. 2018;15(1):63-71. doi:10.1021/acs.molpharmaceut.7b00727(IF:4.440)
[17] Meng P, Zhang YF, Zhang W, et al. Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells. Sci Rep. 2021;11(1):40. Published 2021 Jan 8. doi:10.1038/s41598-020-79524-3(IF:4.380)
[18] Li J, Ye W, Xu W, et al. Activation of autophagy inhibits epithelial to mesenchymal transition process of human lens epithelial cells induced by high glucose conditions. Cell Signal. 2020;75:109768. doi:10.1016/j.cellsig.2020.109768(IF:3.968)
[19] Li H, Hu X, Cheng C, et al. Ribosome production factor 2 homolog promotes migration and invasion of colorectal cancer cells by inducing epithelial-mesenchymal transition via AKT/Gsk-3β signaling pathway [published online ahead of print, 2022 Jan 28]. Biochem Biophys Res Commun. 2022;597:52-57. doi:10.1016/j.bbrc.2022.01.090(IF:3.575)
[20] Zhang H, Lu Y, Wu B, Xia F. Semaphorin 3A mitigates lipopolysaccharide-induced chondrocyte inflammation, apoptosis and extracellular matrix degradation by binding to Neuropilin-1. Bioengineered. 2021;12(2):9641-9654. doi:10.1080/21655979.2021.1974806(IF:3.269)
[21] Zhao Q, Liu Y, Wang T, et al. MiR-375 inhibits the stemness of breast cancer cells by blocking the JAK2/STAT3 signaling. Eur J Pharmacol. 2020;884:173359. doi:10.1016/j.ejphar.2020.173359(IF:3.263)
[22] Sun Z, Wang Z, Li L, et al. RAGE/galectin-3 yields intraplaque calcification transformation via sortilin. Acta Diabetol. 2019;56(4):457-472. doi:10.1007/s00592-018-1273-1(IF:2.996)
[23] Zhang D, Chen X, Zheng D. A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells. Appl Biochem Biotechnol. 2022;194(5):2061-2076. doi:10.1007/s12010-021-03776-6(IF:2.926)
[24] Zhang F, Ni H, Li X, Liu H, Xi T, Zheng L. LncRNA FENDRR attenuates adriamycin resistance via suppressing MDR1 expression through sponging HuR and miR-184 in chronic myelogenous leukaemia cells. FEBS Lett. 2019;593(15):1993-2007. doi:10.1002/1873-3468.13480(IF:2.675)
[25] Pan T, Qian Y, Li T, et al. Acetyl l-carnitine protects adipose-derived stem cells against serum-starvation: regulation on the network composed of reactive oxygen species, autophagy, apoptosis and senescence. Cytotechnology. 2022;74(1):105-121. doi:10.1007/s10616-021-00514-y(IF:2.058)
[26] Li X, Zhang N, Zhang Y, et al. E3 ligase Fbw7 participates in oxidative stress‑induced myocardial cell injury via interacting with Mcl‑1. Mol Med Rep. 2019;20(2):1561-1568. doi:10.3892/mmr.2019.10394(IF:1.851)