本Alexa Fluor 350标记山羊抗兔IgG (H+L)(Alexa Fluor 350-labeled Goat Anti-Rabbit IgG (H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 350是一种常用的非常明亮的蓝色荧光探针。Alexa Fluor 350的荧光光谱与DAPI和Hoechst比较接近。Alexa Fluor 350的吸收(激发)和发射峰参见下表。
Fluorophore |
Absorption Peak (nm) |
Emission Peak (nm) |
Alexa Fluor 350 |
346 |
442 |
本抗体为用纯化的兔IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、山羊IgG、牛IgG
(bovine IgG)、小鼠IgG和大鼠IgG吸附纯化的优质二抗。对人IgG、山羊IgG、牛IgG(bovine IgG)、小鼠IgG和大鼠IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。
本Alexa Fluor 350标记山羊抗兔IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:
产品编号 |
产品名称 |
包装 |
A0408 |
Alexa Fluor 350标记山羊抗兔IgG (H+L) |
100μl |
— |
说明书 |
1份 |
保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用本产品的文献:
1. Wei Shi, Shuge Sun, Yu Han, Yu Tang, Weishang Zhou, Xueying Du, Guangxu Liu
Microplastics impair olfactory-mediated behaviors of goldfish Carassius auratus
J Hazard Mater. 2021 May 5;409:125016.;doi: 10.1016/j.jhazmat.2020.125016. (IF 9.038)
2. Hu T, Li SS, Lu MN, Zhang L, Chen B, Mao R, Mei R, Tan YX, Li S, Xiyang YB
Neuroprotection induced by Navβ2‑knockdown in APP/PS1 transgenic neurons is associated with NEP regulation.
Mol Med Rep. 2019 Aug;20(2):2002-2011. (IF 2.1)
3. Guo L, Li Y, Tian Y, Gong S, Chen X, Peng T, Wang A, Jiang Z
eIF2α promotes vascular remodeling via autophagy in monocrotaline-induced pulmonary arterial hypertension rats.
DRUG DES DEV THER. 2019 Aug 13;13:2799-2809. (IF 3.216)
4. Ding KK, Yang F, Jiang HQ, Yuan ZQ, Yin LL, Dong LY, Cui W, Gou Q, Liu XD, Wu YM, Jiang XY, Zhang X, Zhou PK, Yang CJ
Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells.
Oncol Lett. 2019 Sep;18(3):2704-2711. (IF 2.311)
5. Shangwen Xiao, Bin Song
LncRNA HOXA-AS2 promotes the progression of prostate cancer via targeting miR-509-3p/PBX3 axis
BIOSCIENCE REP. 2020 Aug 28;40(8):BSR20193287.;doi: 10.1042/BSR20193287. (IF 2.942)
6. Xinyu Bian, Dongmin Shi, Kailin Xing, Hongxin Zhou, Lili Lu, Dahai Yu, Weizhong Wu
AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation
Clin Transl Med. 2021 Mar;11(3):e352.;doi: 10.1002/ctm2.352.
7. Yu Yao, Dongxiao Qu, Xiaoping Jing, Yuxiang Jia, Qi Zhong, Limin Zhuo, Xingxing Chen, Guoyi Li, Lele Tang, Yudan Zhu, Xuemei Zhang, Yonghua Ji, Zhiping Li, Jie Tao
Molecular Mechanisms of Epileptic Encephalopathy Caused by KCNMA1 Loss-of-Function Mutations
Front Pharmacol. 2022 Jan 13;12:775328.;doi: 10.3389/fphar.2021.775328. (IF 4.225)
8. Kailu Wang, Pengfei Cui, Ruiqi Ni, Huiling Gong, Hao Li, Wenjun Yan, Xue Fu, Liang Chen, Changwei Lei, Hongning Wang, Xin Yang
Chicken-Derived Pattern Recognition Receptor chLGP2 Inhibits the Replication and Proliferation of Infectious Bronchitis Virus
Front Microbiol. 2022 Jan 25;12:810215.;doi: 10.3389/fmicb.2021.810215. (IF 4.235)