α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001)

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产品编号 产品名称 产品包装 产品价格
AF0001 α-Tubulin Rabbit Polyclonal Antibody 100μl 448.00元
来源 用途 交叉反应性 分子量
Rabbit WB, ICC, IHC, IF H, M, R ～50KDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB IP IF IHC ICC FC ELISA ChIP
1:1000 – 1:100 1:50 1:50 – – –

抗体详细信息如下:

About this Antibody

Name

α-Tubulin Rabbit Polyclonal Antibody

Category

Polyclonal antibody (pAb); Primary antibody

Isotype

IgG

Purification

Affinity purification

About the Immunogen

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.

Gene ID

7277

SwissProt

P68366

Synonyms

ALS22;TUBA1;H2-ALPHA

Category

Cell Cycle/Organism- specific biosystem

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

包装清单：

产品编号 产品名称 包装
AF0001 α-Tubulin Rabbit Polyclonal Antibody 100μl
AZ100 Western一抗稀释液 100ml
— 说明书 1份

保存条件：
α-Tubulin Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。 
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:

α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001) α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001)

Fig. 1. Western blot analysis of cell lysates from NIH/3T3 (1) and HepG2 (2) using α-Tubulin rabbit polyclonal antibody (AF0001).
α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001)
Fig. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using α-Tubulin rabbit polyclonal antibody (AF0001).
α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001)
Fig. 3. ICC staining of α-Tubulin in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilized with PBS containing 0.25% Triton X-100 and counterstained with DAPI to visualize the nucleus (blue).

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α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001)
α-Tubulin Rabbit pAb(α-Tubulin兔多抗)(AF0001)

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