DsRed Tag Mouse mAb(DsRed Tag 小鼠单抗)(AF2906)
产品编号 | 产品名称 | 产品包装 | 产品价格 |
AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl | 299.00元 |
来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
Mouse | WB, IP, IF, IHC, ICC | DsRed-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
1:500-1:2000 | 1:50 | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
About this Antibody | |
Name | DsRed Tag Mouse Monoclonal Antibody |
Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
Isotype | IgG1 |
Purificationmethod | Affinity purification |
Positive samples | – |
Cellular location | – |
Customer validation | – |
About the Immunogen | |
Immunogen | Recombinant DsRed protein expressed in E. coli |
Sequence | – |
Gene ID | – |
SwissProt | – |
Synonyms | – |
Category | Polypeptide protein tag |
Background | DsRed is a red fluorescent protein cloned by homology to Green Fluorescent Protein (GFP) from Discosoma coral. DsRed exhibits excellent resistance to pH extremes and photobleaching and causes a strong red-shift from 558 nm to 583 nm once matured. Immature DsRed shows GFP-like excitation and emission maxima. The contrast created through GFP and DsRed spectral shifts allows for a powerful dual reporter system. |
包装清单:
产品编号 | 产品名称 | 包装 |
AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50µl |
AZ050 | Western一抗稀释液 | 50ml |
— | 说明书 | 1份 |
保存条件:
DsRed Tag Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
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