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VGAT (vesicular GABA transporter, VIAAT)
GABA transporter in the membrane of synaptic vesicles
Fact Sheet Cat. No. 131 002
Polyclonal rabbit antiserum, antibody 1 cytoplasmic domain | |
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Cat. No. 131 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
Applications | WB: 1 : 1000 up to 1 : 5000 (AP staining, see remarks) blot IP: yes ICC: 1 : 100 up to 1 : 1000 image IHC: 1 : 500 up to 1 : 3000 image IHC-P: yes |
Immunogen | Synthetic peptide AEPPVEGDIHYQR (aa 75 – 87 in rat) coupled to key-hole limpet hemocyanin via an added N- terminal cysteine. |
Reactivity | Human, rat, mouse, monkey; other species not tested yet. |
Specificity | Specific for VGAT (K.O. verified). |
Remarks | WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only withnon-boiled samples. |
VGAT(水泡GABA转运蛋白,VIAAT)
突触小泡膜中的GABA转运蛋白
一般信息
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as thevesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
相关产品信息如下:
Cat. No. | Antigen | Product | Applications | Clone | Qty. |
131 011 | VGAT cytoplasmic domain |
mouse monoclonal, purified IgG, unlabeled K.O. verified |
WB IP ICC IHC IHC-P EM | 117G4 | 100µg |
131 011C2 | VGAT cytoplasmic domain |
mouse monoclonal, purified IgG, Oyster 488-labeled K.O. verified |
ICC | 117G4 | 100µg |
131 011C3 | VGAT cytoplasmic domain |
mouse monoclonal, purified IgG, Oyster 550-labeled K.O. verified |
ICC IHC | 117G4 | 100µg |
131 011BT | VGAT cytoplasmic domain |
mouse monoclonal, purified IgG, biotin-labeled K.O. verified |
WB IP ICC IHC | 117G4 | 100µg |
131 002 | VGAT cytoplasmic domain |
rabbit polyclonal, antiserum antibody 1, K.O. verified |
WB IP ICC IHC IHC-P | 200µl | |
131 003 | VGAT cytoplasmic domain |
rabbit polyclonal, affinity purified antibody 1, K.O. verified |
WB IP ICC IHC EM | 50µg | |
131 006 | VGAT cytoplasmic domain |
chicken polyclonal, purified IgY | ICC IHC IHC-P | 50µg | |
131-0P | VGAT cytoplasmic domain |
control peptide | 100µg | ||
131 013 | VGAT cytoplasmic domain |
rabbit polyclonal, affinity purified antibody 2, K.O. verified |
WB IP ICC IHC EM | 50µg | |
131 004 | VGAT cytoplasmic domain |
Guinea pig polyclonal, antiserum, K.O. verified | WB ICC IHC IHC-P EM | 100µl | |
131-0GP | VGAT cytoplasmic domain |
control protein | 100µg | ||
131 103 | VGAT lumenal domain |
rabbit polyclonal, affinity purified, unlabeled K.O. verified |
WB IP ICC IHC | 50µg | |
131 103C2 | VGAT lumenal domain |
rabbit polyclonal, affinity purified, Oyster 488-labeled K.O. verified |
ICC IHC | 50µg | |
131 103C3 | VGAT lumenal domain |
rabbit polyclonal, affinity purified, Oyster 550-labeled K.O. verified |
ICC IHC | 50µg | |
131 103C5 | VGAT lumenal domain |
rabbit polyclonal, affinity purified, Oyster 650-labeled K.O. verified |
ICC IHC | 50µg | |
131 103CpH | VGAT lumenal domain |
rabbit polyclonal, affinity purified, CypHer5E-labeled K.O. verified |
ICC IHC | 50µg |