haemtech Thrombin (alpha) 说明书

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Haematologic Technologies Inc. (HTI) 公司是一家专门致力于高质量的人血浆蛋白的分离和特性研究的重要生产商, 其产品非常适用于体内研究。在公司发展的第二个十年里,HTI将目光投向参与血液凝集和纤维蛋白溶解,以及骨代谢调节的蛋白的研究。HTI产品系列含有超 过100种高纯度和特异性的蛋白,包括:酶原、酶、协同因子和抑制剂,同时还提供补体系列的单克隆和多克隆抗体。另外,HTI还提供一些技术服务,如:蛋 白定制、纯化、修饰、科研合作等。

 

Thrombin (alpha)

凝血酶
蛋白水解激活丝氨酸蛋白酶α-凝血酶是通过酶原,凝血酶原的蛋白水解活化产生的。酶复合物凝血酶原酶催化凝血酶原中两个肽键的蛋白水解,其产生NH2-末端衍生的F1.2区和异源二聚体α-凝血酶。α-凝血酶由通过单个二硫键与“B”链(Mr = 31,000)共价连接的“A”链(Mr = 6000)组成。

 

 

 

 

 

 

货号:HCT-0020 

Human alpha-Thrombin

Sizes 100 µg, 1 mg, 10 mg (1 mg vials)
Formulation 50% glycerol/water (v/v)
Storage -20°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:HCT-DFP 

Human alpha-Thrombin, DFP active site blocked

Size 100 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:HCT-FPRCK 

Human alpha-Thrombin, PPACK (FPRck) active site blocked

 

Size 100 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:HCT-BFPRCK 

Human alpha-Thrombin, Biotinylated PPACK (FPRck) active site blocked

Size 100 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:BCT-1020 

Bovine alpha-Thrombin

 

Sizes 200 µg, 1 mg
Formulation 50% glycerol/water (v/v)
Storage -20°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

货号:BCT-DFP 

Bovine alpha-Thrombin, DFP active site blocked

 

Size 200 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:BCT-FPRCK 

Bovine alpha-Thrombin, PPACK (FPRck) active site blocked

 

Size 200 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:BCT-BFPRCK 

Bovine alpha-Thrombin, Biotinylated PPACK (FPRck) active site blocked

 

Size 200 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:MCT-5020 

Mouse alpha-Thrombin

 

Size 50 µg
Formulation 50% glycerol/water (v/v)
Storage -20°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

凝胶 Novex 4-12%Bis-Tris
加载 人类β和γ凝血酶,每泳道1μg
缓冲 MES
标准 见蓝皮书2; 肌球蛋白(188kDa),磷酸化酶B(98kDa),BSA(62kDa),谷氨酸脱氢酶(49kDa),醇脱氢酶(38kDa),碳酸酐酶(28kDa),肌红蛋白红(17kDa),溶菌酶kDa),抑肽酶(6kDa),胰岛素,B链(3kDa)。