Histone-H3小鼠单克隆抗体 Histone-H3 Mouse mAb
产品说明书
FAQ
COA
已发表文献
组蛋白是真核生物中负责染色体纤维核小体结构的基本核蛋白。核小体由大约146 bp的DNA包裹在由四个核心组蛋白(H2A、H2B、H3和H4)每对组成的组蛋白八聚体上。染色质纤维通过连接蛋白H1与核小体之间的DNA相互作用进一步致密，形成更高阶的染色质结构。该基因无内含子，编码一个依赖复制的组蛋白，是组蛋白H3家族的成员。该基因的转录本缺少polyA尾部;相反，它们包含回文终止元素。该基因与6p22-p21.3染色体组蛋白基因集群中的其他H3基因是分开的。
产品性质
|
别名 |
H3 histone, HIST1H3A, Histone cluster 1, H3a |
|
SwissProt ID |
P68431 |
|
基因ID |
8290 |
|
免疫原 |
Purified recombinant protein fragments expressed in E.coli. |
|
缓冲液 |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
|
分子量 |
Observed MW (kDa):15 |
|
克隆类型 |
Monoclonal Antibody |
|
亚型 |
IgG1 |
|
宿主 |
Mouse |
|
物种反应 |
Human,Mouse,Rat,Yeast |
|
纯化方式 |
Affinity Purified |
|
细胞定位 |
Chromosome,Nucleus |
|
研究领域 |
– |
应用
稀释比 WB: 1/2000-5000 ICC: 1/100-500 IP: 1/200 IHC: 1/50-300
 |
Western blot analysis of extracts from C6,3T3 and MCF7 cell lysates using Histon H3 Mouse mAb (1:20000 diluted).Predicted band size:17KDa.Observed band size:17KDa. |
|
|
IF analysis of Hela with DAPI (Left) and (Right) diluted at 1:200
|
|
|
1 、 Input: Hela Cell Lysate 2 、 IP product: IP dilute 1:20 Western blot analysis: primary antibody : 1:2000,. Secondary antibody: Goat anti-Mouse IgG(H+L) (S1) 1:10000
|
|
|
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H3 Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tem- peRature, 30min). Negative control was used by secondary antibody only. |
|
|
Western blot detection of Histone H3 in C6,3T3,Hela cell lysates using Histone H3 Mouse mAb(1:10000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
|
 |
Immunoprecipitation analysis of Hela cell lysates using Histone H3 Mouse mAb
|
运输与保存方法
冰袋运输。-20℃保存，1年有效期。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
HB220529
组蛋白是真核生物中负责染色体纤维核小体结构的基本核蛋白。核小体由大约146 bp的DNA包裹在由四个核心组蛋白(H2A、H2B、H3和H4)每对组成的组蛋白八聚体上。染色质纤维通过连接蛋白H1与核小体之间的DNA相互作用进一步致密，形成更高阶的染色质结构。该基因无内含子，编码一个依赖复制的组蛋白，是组蛋白H3家族的成员。该基因的转录本缺少polyA尾部;相反，它们包含回文终止元素。该基因与6p22-p21.3染色体组蛋白基因集群中的其他H3基因是分开的。
产品性质
|
别名 |
H3 histone, HIST1H3A, Histone cluster 1, H3a |
|
SwissProt ID |
P68431 |
|
基因ID |
8290 |
|
免疫原 |
Purified recombinant protein fragments expressed in E.coli. |
|
缓冲液 |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
|
分子量 |
Observed MW (kDa):15 |
|
克隆类型 |
Monoclonal Antibody |
|
亚型 |
IgG1 |
|
宿主 |
Mouse |
|
物种反应 |
Human,Mouse,Rat,Yeast |
|
纯化方式 |
Affinity Purified |
|
细胞定位 |
Chromosome,Nucleus |
|
研究领域 |
– |
应用
稀释比 WB: 1/2000-5000 ICC: 1/100-500 IP: 1/200 IHC: 1/50-300
 |
Western blot analysis of extracts from C6,3T3 and MCF7 cell lysates using Histon H3 Mouse mAb (1:20000 diluted).Predicted band size:17KDa.Observed band size:17KDa. |
|
|
IF analysis of Hela with DAPI (Left) and (Right) diluted at 1:200
|
|
|
1 、 Input: Hela Cell Lysate 2 、 IP product: IP dilute 1:20 Western blot analysis: primary antibody : 1:2000,. Secondary antibody: Goat anti-Mouse IgG(H+L) (S1) 1:10000
|
|
|
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H3 Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tem- peRature, 30min). Negative control was used by secondary antibody only. |
|
|
Western blot detection of Histone H3 in C6,3T3,Hela cell lysates using Histone H3 Mouse mAb(1:10000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
|
 |
Immunoprecipitation analysis of Hela cell lysates using Histone H3 Mouse mAb
|
运输与保存方法
冰袋运输。-20℃保存，1年有效期。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
HB220529
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